Case: Homicide or suicide?

Qualitative analysis:

Mesaconitine, Aconitine and Jesaconitine identified in deceased's urine sample.

(IDENTITY APPROVED)









Quantitive analysis: 
Calculation of concentration of Mesaconitine, Aconitine, Jesaconitine in Urine sample

We calculate the (Ratio of standard aconitines/ Internal standard) by using the peak areas. With this results, we will be able to plot the calibration graph (Ratio of standard aconitines/ Internal standard against concentration of each of the Aconitines (Mesaconitine, Aconitine, Hypaconitine, Jesaconitine) by using the 3 standards.

After the calibration graph is plotted, we will have  linear equations for each of the 4 aconitines. This equation will be used to calculate the concentration of Mesaconitine, Aconitine, Jesaconitine in the deceased's urine sample. Thus, we will be able to quantitate trace amount of any of the aconitines present in the urine sample, and determine which is the highest concentration as well.