LC-MS instrument to detect Aconitine in urine sample
HPLC: Waters 2690 instrument
XTerra RP18 column: 150 x 2.1-mm i.d., 3.5-µm particle size
Guard column: 10 x 2.1-mm i.d.
Injection volume: 20µl
Mobile phase: (24 parts : 76 parts, v/v)
0.1% (v/v) formic acid in acetonitrile
0.1% (v/v) formic acid in aqueous solution
Flow Rate: 0.2mL/min
Column temperature: 40 oC
MS: Micromass ZMD 4000 instrument (Waters)
Mode: electrospray ionization positive ion
Parameters:
capillary potential 3.0 kV
cone voltage 60V
ion source temperature: 120 oC
drying gas temperature: 350 oC
nitrogen gas flow rate: 350 L/h
Measurements: selected ion monitoring mode
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